anti darpp32 rabbit cell signaling  (Cell Signaling Technology Inc)

Journal: Frontiers in Aging Neuroscience

Article Title: Abnormal outer and inner retina in a mouse model of Huntington’s disease with age

doi: 10.3389/fnagi.2024.1434551

Figure Lengend Snippet: Information of antibodies used in Western blotting.

Article Snippet: Rabbit monoclonal anti-Darpp32 , Cell signaling, Danvers, Massachusetts, USA , 2306S , , 1:1000 , Brain.

Techniques: Western Blot

Journal: Frontiers in Aging Neuroscience

Article Title: Abnormal outer and inner retina in a mouse model of Huntington’s disease with age

doi: 10.3389/fnagi.2024.1434551

Figure Lengend Snippet: Information on antibodies used in immunofluorescent staining.

Article Snippet: Rabbit monoclonal anti-Darpp32 , Cell signaling, Danvers, Massachusetts, USA , 2306S , , 1:1000 , Brain.

Techniques: Staining, Electron Microscopy

Journal: Journal of Parkinson's Disease

Article Title: Serotonergic Regulation of Synaptic Dopamine Levels Mitigates L-DOPA-Induced Dyskinesia in a Mouse Model of Parkinson’s Disease

doi: 10.3233/JPD-240080

Figure Lengend Snippet: The expression of proteins involved in dopaminergic and serotonergic transmission was analyzed in the striatum of WT, MP, and MP LID mice following co-treatment of L-DOPA with 5-HTP or Citalopram. A) Dopaminergic and serotonergic transmission-related protein expression in the striatum were illustrated. B) There was no significant change in D1 receptor protein expression among these groups. However, the administration of 5-HTP effectively suppressed the increased phosphorylation of signaling molecules in the downstream D1 pathway, specifically ERK (red bar: MP LID + Saline + L-DOPA vs. blue bar: MP LID + 5-HTP + L-DOPA, p < 0.01) (C) and DARPP32 ( p < 0.01) (D), in the MP LID mice. In contrast, the administration of Citalopram did not have a significant effect on the phosphorylation levels of these molecules. There were no significant changes in the expressions of 5-HT1A receptor (E) and 5-HT1B receptor (F) proteins among these groups. One-way ANOVA followed by a Bonferroni post hoc test for multiple comparisons. ** p < 0.01, compared to MP LID + Saline + L-DOPA; MP compared to MP LID + Saline + L-DOPA, ## p < 0.01.

Article Snippet: Following a 45-min incubation in blocking buffer (2% bovine serum albumin and Tris-buffered saline with Tween 20), the membranes were subjected to overnight incubation with primary antibodies: D1 R (1 : 1000, rabbit, GTX100354, GeneTex, Taiwan), pERK (1 : 1000, rabbit, #3179, Cell Signaling Technology, Danvers, MA, USA), ERK (1 : 1000, rabbit, #9102, Cell Signaling Technology, Danvers, MA, USA), pDARPP32 (1 : 1000, rabbit, #5393, Cell Signaling Technology, Danvers, MA, USA), DARPP32 (1 : 1000, rabbit, #2306, Cell Signaling Technology, Danvers, MA, USA), 5-HT1A (1 : 1000, rabbit, GTX02545, GeneTex, Taiwan), 5-HT1B (1 : 1000, rabbit, NB100-56350, Novus, Centennial, CO, USA), and β-actin (1 : 5000, rabbit, ab8227, Abcam, Cambridge, United Kingdom).

Techniques: Expressing, Transmission Assay, Phospho-proteomics, Saline